It’s a new year, and a new project

MikeK

 

Well, as we were saying.

Every field season brings new people and new opportunities. This year, the Kaspari lab from the University of Oklahoma teams up with the Kay lab of St. Thomas University in Minnesota, to bring you another set of field notes from Panama. 

One of our goals this summer is to begin to understand the chemical “recipe” of the ants of Barro Colorado Island. Turns out, that while we are all made of elements like carbon, nitrogen, phosphorus, and potassium (the latter three representing the N, P, and K numbers found on any bag of fertilizer), subtle differences in the elemental recipe of ants may be important in determining which species grow fast (we think it’s the phosphorus), run around like whirling ant dervishes (we think it’s the carbon), or lumber around in the heavy army of chitin (the thing that gives a beetle it’s distinctive “crunch”, and we think *that’s* nitrogen). 

In order to do this, we need lot’s of ants. Specifically, we need to grovel through the litter looking for whole ant colonies, but we also need to collect the winged reproductives of ant colonies–the males and females that fly from their parent colony to mate. This, we think, is a crucial time in an ant’s life that may be *especially* dependent on its chemical recipe. 

Which explains why the tip of my nose is sunburned.

Bear with me on this one.

One great way of catching flying insects, at least the nocturnal ones, is to blacklight. A blacklight is basically a light bulb that gives off UV light. For reasons that are not entirely clear, many insects are drawn inexorably to the ultraviolet light given off by a blacklight.

BlacklightingBCI So a couple of days after I arrived on the island (about 10 days ago) I was standing in front of a while plastic tarp, with a pair of forceps (=tweezers), while insects buzzed, fluttered, and thwapped around me, some hitting the tarp and falling to the ground, others hanging on, dazed by the light, while others skittered around. The “skittering” variety tended to be what I was after, the winged ants who rarely stick around, but carom off the tarp and head back out to the night. These ants were not going to make easy, and I felt slow and clumsy, with my right tweezer-laden hand stabbing out like a drunken heron going after minnows.

BlacklightingBCI2

The UV light, didn’t help frankly. That was one strange, disorienting bulb. But eventually I had captured about 20 alates, and felt reasonably good about the night’s catch, as the dry season had not ended yet, and its the *wet* season that really brings out the ants. More on that later. So I plunked my ziplock full of alate into the freezer and called it a night.

So, about the nose.

Debby, my wife, a crackerjack artist, and fellow blogger, stared at me the next day, screwed up her face and said, “what did you do to your nose?”  Not that I look very closely at the tip of my nose with any regularity. Its rather awkward to do so frankly. But I headed to the bathroom mirror and there, like a bright little penny, was a red spot.

Apparently pressing your face up against a UV bulb ain’t a particularly good idea.

Obviously, we are going to have to work on this blacklighting protocol.

Each star is a setting sun.

Tonight I’m not feeling particularly poetic, so this post may be lacking flare and pizzaz in respects to avoid sentiment or emo-talk.

It’s our last night on the island. Matt is scraping up the last of his data, I’m staring at my $5 bottle of white wine, counting down the minutes until I pop the cork and put my feet up. It felt really good to type in all my data I collected from Litter Piles 2006. I’m anxious to get back to the lab, pull out the scope and sort my whirl packs. I hope I will have significant results.

The trip started with me reading “On Becoming a Biologist” by John Janovy Jr., which Mike bestowed upon me before the the trip. This book is an honest and blunt interpretation of being in academia as a biologist, and delves a little into the mind of a biologist:

” With the recognition of desire as a driving force, we begin to get a clue as to what motivates satisifed biologists: They love what they are doing. More often than they will admit, their love is for the organisms themselves.”

- John Janvoy Jr., page 36

I have learned at least three very significant aspects of field biology from this trip:

  1. Experience designing experiments and learning from errors with design.
  2. How to cope with monotonous and repetitive lab work, and focusing on the gold pot at the end of the rainbow: DATA
  3. Developed a love for an organism, a desire to know, an urge to explore.

Since I am at the beginning of my journey towards graduate school, I keep my senses alert for a topic of study. I knew before I came that fungal parasites interest me, but I didn’t have a clue what I really wanted to spend a large portion of my life masticating. Now, I think I know: FUNGI. I love how it digests externally by spewing out enzymes. I love how you can only see it when it’s reproducing. I love the wide variety of fungi and the unique fruiting bodies that result. I love how it has applicable uses for human health. I love how it hides in leaves as an endophyte, grows tall, hard fruiting bodies on decomposing logs on the rainforest trail, manipulates and uses arthropods, and how it can even be a parasite on another species of fungi.

One thing I anticipate may prove to be a road block is proving I am as qualified to be a successful scientist as my male counterparts. In last Friday’s Nature, a commentary was published criticising three scientific hypotheses that women are handicapped inately by our brain structure; this is why women are trailing men in science. Ben Barres draws from three scientists: Peter Lawrence, Larry Summers, and to my surprise, Steven Pinker. Although, I do believe there are differences between men and women, they are not brain related as much as hormonal. I am just as competitive, intelligent, ambitious, and dedicated as any male can be. (so there!)

If you are contemplating an education in field biology, my advice is to stop thinking and start doing. It’s the only way I was able to conceive what this type of work entails and develop an understanding of the type of life I would be chaining myself to for the duration of my scientific inquiries.

Bon voyage!! I really had a killer time.

From one chord to another…

As a student at The Ohio State’s summer “Soil Acarology Course,” this has been my state for the last week:

“Dorsosejugal scissure with 3 arches. Octotaxic system reduced to 4 pairs of very small saccules. On palptarsus eupathidium, acm and solenidion on large apophysis and strongly curved…………….Rostrozetes”

A couplet from a key. The mite course goes something like this. Arrive at lab 830AM. Identify mites until 1030PM. Pretty intense. This is precious knowledge. Only a handful of people in the world know how to look for and look at these tiny arthropods that cover nearly every surface on the planet. Today, we will nearly be finished learning the families of Mesostigmata. We even sorted through July 4th fireworks–granted many of the folks in the class are not Americans–but still, we could hear pounding of the stuff in the sky outside the windowless room.

I realize this note postdates my time on BCI, but I am a night writer and my nights on the island were busy. No, not in that way! I was taking care of ca. 60 ant colonies as part of research investigating why tropical litter ant colonies mature at such small sizes. Amazingly, tropical forests are home to some of the world’s largest (the leaf cutters and army ants) and smallest ant colonies. As a lover of small things, I have become fascinated by twig inhabiting colonies of ants that approach densities of 5 colonies per meter squared but would never be observed while casually hiking.

An ant colony experiences periods of growth, during which the queen produces sterile workers. At various periods during its development, the colony reproduces as the queen produces sexual winged males and females that fly away and mate with sexual ants from other colonies. While many colonies in temperate zones often do not approach “sexual maturity” until they contain 1,000’s of workers, tropical litter colonies routinely reproduce with only 50 workers! By raising colonies in captivity under various levels of resource availability, I sought to understand how food limitation influenced colony life histories (e.g. growth rates and size at maturity).

My project was not without its struggles. During my first BCI month, I refined my culturing techniques—learning what conditions my ants liked and emphatically disliked. For instance, one night after about a month of painstakingly establishing colonies, I made the fateful decision to change their diets to cold-cut turkey. This attempt to standardize supplemented protein unfortunately initiated a bacterial outbreak which killed half my colonies over night. I experienced something a bit beyond chagrin when I saw the last of the living ants writhing in agony. Some times fungi overtook the jell-o diet I had lovingly prepared, while at others, escaped ants searched the lab table for suitable habitat.

Last summer, I sampled insects from meter squared plots in leaf litter, extracting critters in alcohol and then spending the next 6 months sorting them dead under a scope. This summer, I was determined to “get to know” the soil organisms in a more dynamic way. It was wonderfully pleasant to spend late nights in the soil lab, watching ants actively behaving. Each colony, as a “superorganism,” seemed to have its own personality, and as a scientist, I had to refrain from assigning them names! Some routinely foraged en masse, while some subtly sent out major workers. Some organized their garbage into neat piles while others left detritus haphazardly strewn about the brood chambers. They all seemed to wage pitched battles against fungi, and I took it upon myself to serve as their maid, frequently cleaning out their homes.

The island seems a bit distant now, as I have immersed myself in this mite business. Soil mites are a bit frustrating to identify through morphological characters because, being basically blind and tiny, visual cues are not part of their lives in the peacock feather sense. Mite specimens are also usually mounted as crushed pancakes on slides, with heads as fragmented pieces. To the patient observer, however, they are beautiful variations on armored themes. To litter ants they are thought to be breakfast lunch and dinner! But such dynamics will remain fodder for future versions of my research…

Best of luck to Matt and Risa who are still on BCI. I hope they drink a beer on the balcony for me!

A week is all we have …

My family is already counting down the days until my return. E-mail messages with subject headings of homecomings and last-minute summer activities. I suppose I have missed out on a big block of baseball games, mosquito bites, 100 degree weather, tornado warnings and friendly get-togethers. With a week left until my return to the United States, I am feeling the tolls of long days in the field, late nights reading, socializing and computing data.

Today I made my last long trek into the BCI field, heavy bags in hand, to harvest my transects. I sifted 40 transects and set up 54 Berlese funnels before dinner. I felt sad, as if a long hike up a hill had suddenly plateued into a misty haze, as if collecting isopods, watching them scurry across filter paper — my frustrated eyes willing them towards apples — my desires abruptly fell into alcohol … it’s almost over and I wonder if I’ve achieved anything with my efforts or simply skipped down trails and gazed in amazement. It’s also discerning to think I have developed a compassion, attachment, maybe … love for little crustaceans who occupy the litter with snap-jaw ants and white hyphee decorations simply to watch them expire into quantified data.

Tomorrow Laura will return to Holland, tonight a small portion of BCI bar babes will send her off, and I will try not to imagine myself in her place on the last boat leaving the island for a new destination until next week when I will see the balcony from a distance, slowly becoming more desert as summer field season 2006 comes to a close.

Stardate: July 11th…

The great decomposition experiment on the Gigante fertilization plots has all been picked up. Risa and I are busy in the throes of washing all the hemicellulose and lignin so it can be dried and later weighed to determine decomposition rates in the litter over the last month or so.

The island has been getting more and more quiet in the last couple of weeks. Many of the scientists and students who arrived after we did have already left, but for us the work continues. It’s actually pretty nice. Tonight on the lounge patio a group of five spider monkey males ambushed three of us as we sat sipping beers. They evidently thought there might be food in the trash cans and had come to check it out. Finding it empty, the left quickly, but for me, the day had been made: accosted by spider monkeys–this place is ripe for great stories. One of the ‘long-time’ student residents, Mariah Hopkins, left the island for good this evening. Having finished the field portion of her Ph. D work on howler monkeys, she is returning to Berkeley. Laura, Christian, and I sent her off on the late boat with a pathetic imitation of a howler monkey chorus–god it must have been awful.

Risa and I both need to harvest our field work towards the end of this week. Since last Saturday, the work here has been a large ‘mop-up’ action: data is being gathered, harvested, and cataloged so we can return to Oklahoma. Perhaps, as we finish next week, someone will give a rousing send-off with…an ant-like…something…

Time is of the essence.

Since Mike left, I have been making the best out of the BCI experience. I went on a recreational trail walk with Betsy Arnold where she pointed out some of the rainforest’s treasures. We almost stepped on a coral snake, held a frog that looks like a leaf, and gazed at the Panama Canal from a clearing at the end of the trail. Early one morning, before the sun gracefully showered through the canopy, I accompanied a spider monkey researcher on the hunt for urine. I saw tamarinds, peccaries, howlers and learned when off trail, anything looks like it could be a trail.

This weekend my lab mate Matt and I will cruising to Gigante to battle uncertain weather to pick up the last of Mike’s decomposition experiment. We will inevitably disappear from the social circles of BCI, cleaning, drying and weighing until Harry Potter’s adventures are exhausted and my entire iTunes collection has become as exciting as opening the last present on Christmas morning in 1985. However, watching the outcome of this experiment unfold is what I look forward to most. I especially enjoy observing the many types of fungi that choose to grow on the filter paper and popsicle sticks.

Soon afterward, we will be leaving the island and returning to reality. This is a place most young scientists dream about while brainstorming experiments and refer to often when hitting the books, taking exams, and driving their cars to work. But before that time can come, I must complete the experiments I have set out on the trails. A couple weeks ago I set up an experiment where I alter the litter depth to observe what elements change. The experiment came to mind while searching unsuccessfully for isopods. I noticed the areas closer to the lab had relatively shallow litter depths and isopods were rare. I thought if I raked up a pile of litter, maybe they would come to me. I was about to set out to do just that very thing when Mike stopped me from half-hazard experimentation. He worked with me to design a transect system where the control and litter pile plots are randomized and running parallel, stretching more than 40 meters. In about a week I will sift the 1/4 square meter plots and Berleze the risidual. The arthorpods collected will be sorted and counted. This will not only give me an idea of how many more isopods are located in contrast to the control plot, but it will also give me a good idea of arthropod diversity in comparison to the two types of plots.I think altering the litter depth will also have an effect on decomposition; although, that waits to be shown.

Earlier I set up a pilot experiment on Fairchild. This mini experiment showed a vast increase in the amount of isopods per a pile plot. I also saw changes in seedlings, fungi, and ant colonies chose to make their home in the leaves. I think Litter Piles 2006 will have an interesting outcome.

In addition, I have a small experiment running in the ambient soil lab. I’m feeding leaves with endophytes to isopods. Recently, I was informed of a bacteria living inside the leaves with the endophytes. It’s unclear yet if the bacteria is a parasite, symbiont, or simply mutually present. I’m curious if the bacteria will be harmful to the isopods or if they will even attempt to munch on endophytic leaves. Even more so, I am absolutely fascinated with the possibilities of the findings of this bacteria and endophyte. There are so many more questions that can arise with the results. It’s also unclear if the endophyte is a parasite; possibly the endophyte is releasing a chemical that makes leaves fall prematurely so it may take over the leaf’s resources sooner and reproduce. I hope to at some point discover my own answers to questions dealing with fungi. In fact, I have already started reading and seeking the answers. I think it’s best to behave like a Ph.D. student now and a get a head start on the game.

Time is on our side…

Last summer was my first on BCI, and in many ways it will never be the same here as in that first field season. As a newbie, I wasn’t privy to every inside joke of the island, and much of the social craziness that seems to accompany this place slipped by me unnoticed. This year, I’ve felt more in touch with the people, as I more and more think of this place as a small town where everyone knows your name…and your business. My business this summer has been about ants. I think this is the summer that I’ll look back and say “I knew I wanted to study ants one summer on BCI when…”

Has everything been perfect with my research? Not exactly, but I’ve learned a lot, and I feel like I’m one step closer to being ready for a great graduate school experience. We recently finished a harvest of half of Mike’s great field project this season on gigante. I also spent a day in town getting a boat license to operate a boat on the Panama Canal. Risa and I both have sample plots and transects to be tended, and an outdoor laboratory of fungi experiments. I’m also collecting pilot data for a project involving Azteca ants. In my experience, life in the last two weeks on BCI can be really hectic, but I’m trying to remember to stop a smell the roses, figuratively. A recent heavy rain brought out the toads and frogs in masse, so I grabbed a headlamp and headed up to Kodak house. In back, there is a small frog pond, where I finally spotted some red-eyed tree frogs. It felt good to first identify them by their call, and then to finally spot them. It was also good to pause and reflect on how much I enjoy just stopping to look at the forest.

I think I’ve made a new observation on the army ants here (Eciton burchellii) that hasn’t been seen before. If so, it’s a good reminder that I’m doing what I’m supposed to here in the forest: keeping my eyes open and making observations. I’ll keep the observation secret for now until I talk it over with Mike and consult the literature. You never know, it could be the next big thing.

A Panama Beer and a glass of Flor de Cana, please.

Tomorrow we say, “so long, jefe” as The Professor boards the mid-day boat to the mainland where we hope he will partake in the infamous margaritas Katie has spoke of at early morning breakfast chats.

It’s been an unforgettable experience working with Mike on BCI. In the field he’s persuaded me to have a keen eye for the little things, a sharp nose for the funky fungi, and a strong will for the difficult things. Some may say sitting with Mike on the balcony, the melodic sounds of howler monkeys through misty haze escaping rainforest tapestry, as cargo ships slide across on the Vaseline waters of Lake Gigante’s croc-swimming moat below, sipping straight 4-year rum from a lounge coffee cup, while prattling about questions remaining unanswered, muttering conversations about the idiocies of our reality, and hearing once again the science story that concludes, “And Risa says, ‘what a dick!’” is the highlight of their interactions with Mike. Others may speak of “lab meeting” drinks in G1, sucking on anchovy-filled green olives, and prying questions from The Prophet Prof are the most precious moments. But the mirror I will predominately choose to reflect takes place in a small, white room that should be padded to muffle the melodic mayhem of yours truly accompanied by none other than Karaoke Kaspari. This fall we’ll be releasing a CD, “The Kaspari-Experience,” (s.r.p., $16.97) featuring our greatest hits like, “Flan Flan Flan, Flan Flan-a-ram” and “White Sheet Hyphee” to the tune of “Soon it will be Christmas Day.”

Tonight there’s a fiesta planned, as one more leaf falls from the BCI island tree, only to be decomposed by various microorganism memories of Field Season 2006. Of course we still have “AntLab Mainland,” broadcasting this fall from the University of Oklahoma campus labs, but our loose footing will be stifled, our dances will be choreographed, and our hearts will be focused on studying from texts, taking notes from lectures, and catching a bite to eat with flash-cards in hand instead of fingering through moist litter and scratching the summer’s chiggers.

Tomorrow, a moment of silence as one more member leaves the AntLab Field Season 2006 cast. Stay tuned as Matt and I compete for Island Monkey Queen (the loser has to carry all the lab bags. I hope I win!).

Confessions of an Antlab lackey…

<phew> Another long but satisfying day harvesting data on the gigante peninsula is over. I considered titling the post “And on the seventh day they rested…” however, I can’t really take credit for the thought. It’s Risa’s—and I hope she will decide to pen a blog post under that title. I’m sure it will be a good one.

I have a confession. When I first joined the lab, I never thought I’d want to study ants long term. My feelings at the time were well in with the characters in John Janovy Jr.’s Dunwoody Pond, who fell into the arms of biology in such a way that “they came and tried to find a small corner in science where they could make a mark. ‘What do you want to work on?’ I’d ask. ‘Something nobody else has worked on’ they replied”.

I suppose I also just wanted to work on something nobody else had. My feelings when I joined Mike’s lab was that ants were attractive to lots of people and the remaining questions (if there were any) would be predictable and hard to get at. What a strange thought this turned out to be in retrospect. What in my collective experience would qualify me to think this and to be so sure of myself? The confidence of the naïve, perhaps? I’m still not sure, but these feelings were my primary motivator at the time. I thought I’d probably work on a really obscure family of beetles or something far off the beaten path. Ah well.

Being new to ants is a strange thing. My interest in insects was at an all-time high when I first started working in the lab, but I had a feeling that the ants were in some way different—outside the range of typical entomologists—and I wasn’t sure I would be a good fit for them. The degree of social cooperation they display combined with the wonderful roles of chemical communication and behavior began taking a toll though, and I have become more and more interested in studying them as time has worn on. Large questions, such as the evolution of eusociality or social behavior, can be tackled with ants.

I’m glad that science is a curved path. I think about Mike and the many hats he has worn over the years—from ornithologist to ant biologist, community ecologist, and perhaps later he’ll even ‘dabble’ in microbial ecology. For me, an interest in insects was only the beginning. I’d enjoy working with ants. At dinner this evening I listened to a grad student lament about searching for coati all afternoon and not seeing one. I’ve never had that problem before…

Fare thee well

One of the delights of the AntLab is the incredible diversity of folks that it attracts to her slate tables, petri dishes full of bugs, and gorgeous views of the side of a building known with no great affection as "the Blender". People from all walks of life come to the AntLab for a place to do a little arthropod sorting, and lots of chatting and hanging out. One never knows what one will hear when you walk in, but the conversation will always be funny and animated.

The AntLab loses one of her own today to the world of dental school. JulieM has been around for what seems like forever to the AntLab oldtimers. Perhaps a bettter way of putting it is that one has difficulty imagining the AntLab without her. In a constellation of individuals, and we mean individuals, Julie was the core around which the neutron stars and red dwarfs circled. She was the one everyone gravitated toward. Although we hate to see her go, we can hardly feel bad about someone moving on to bigger and better things. We just hope Julie takes a bit of the ole' AntLab with her. She has certainly left a lot of herself behind.

So, au revoir Julie, from all your pals in the AntLab. Don't be a stranger, and make sure your practice accept's OU's Dental Plan.

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